Serum or Saliva Extraction of Toxic Compounds from Methyl Methacrylate Dental Materials and HPLC Analysis Combined with SPE

Methyl methacrylate polymer (PolyMMA) is widely used as the composite resin for the dental plate. During the preparation process of PolyMMA by the polymerization reaction, benzoylperoxide (BPO) and N,N’-dimethylp-toluidine (DMPT) are added as the initiator and the stimulator, respectively. These compounds exhibit toxicity as well as a residue potential, their use raises concerns regarding human safety. The degree of elution into serum or saliva was determined to evaluate risk to the user. Analysis was by HPLC combined with solid-phase extraction using a C-18 column. The eluted compounds were found to be in the order of 10 to 70 ppm. *Corresponding author: Shintani H, Faculty of Science and Engineering, Chuo University, 1-13-27, Kasuga, Bunkyo, 112-8551, Tokyo, Japan, Tel: +81425922336; Fax: +81425922336; E-mail: [email protected] Received June 07, 2014; Accepted June 23, 2014; Published June 28, 2014 Citation: Shintani H (2014) Serum or Saliva Extraction of Toxic Compounds from Methyl Methacrylate Dental Materials and HPLC Analysis Combined with SPE. Pharmaceut Reg Affairs 3: 123. doi:10.4172/2167-7689.1000123 Copyright: © 2014 Shintani H. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.